A colorimetric method for the estimation of acetoacetic acid in the blood.
نویسنده
چکیده
It was observed that acetoacetic acid when coupled with 4-nitrobenzenediazonium salt in a buffered carbonate solution yielded a strong green color upon further alkalization. This reaction was given by relatively few of the biochemical compounds that have been tested, and the procedure has been developed so that, as applied to blood filtrates, it is believed t,o afford a. satisfactory measure of acetoacetic acid cont,ent.
منابع مشابه
The estimation of acetone bodies.
In a study of the pathogenesis of bovine ketosis it was felt desirable to be able to follow in some detail the changes in. all three ketone bodies (acetone, acetoacetic acid and ,-hydroxybutyric acid) which have been shown by various workers to occur in excessive quantities in blood, milk and urine during the course of that disorder. Hitherto, little or no attempt hasbeenmade at complete differ...
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Clift and Cook (1) have described a method for the determination of carbonyl compounds in blood as bisulfite-binding substances. Devised primarily for rapid determination of pyruvic acid, the procedure as described made possible the determination of acetoacetic acid to the extent of about 87 per cent, but acetonc could not be measured because of the instability of the addition product. Sufficie...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 179 3 شماره
صفحات -
تاریخ انتشار 1949